Jacqueline HY Siu


I am currently a postdoctoral researcher/ senior bioinformatician at the University of Oxford with Prof Mark Coles and Dr. Calli Dendrou as part of the LEGACY Network. I completed my PhD in the Department of Surgery (Transplantation) at the University of Cambridge in 2021 where I was supervised by Gavin Pettigrew. My doctoral research, funded by a Gates Cambridge scholarship, investigated lymphocyte subset variability between human lymphoid tissues.

Overall, my research combines traditional experimental immunology techniques with computational biology to understand tissue-specific differences in lymphocytes. In particular, I have been using single cell datasets (mass cytometry and 10X) to investigate the immune system in human secondary lymphoid organs during immune homeostasis and vaccination.

I am also passionate about public engagement, STEM outreach, and mentoring. I am currently a Canadian Centennial Scholarship Fund committee member. You can contact me about consulting work and public engagement via email.

Jacqueline Siu

Research Experience

2022 – Present
Oxford, UK

Understanding the immune response upon vaccination in human lymph nodes using ex vivo tissue culture slices and in vivo ultrasound guided fine-needle aspirates
PI: Professor Mark Coles (University of Oxford, Kennedy Institute) and Dr Calliope Dendrou (University of Oxford, Wellcome Centre for Human Genetics)

  • Processed and prepared in vivo samples for acquisition with 10X 5’ VDJ with CITE-Seq antibodies as well as genotyping and tissue typing.
  • Analysed single cell datasets for both the ex vivo and in vivo studies.

2021 – 2022
London, UK

Human marginal zone B cells resolved by global analysis of lymphoid tissues and blood
PI: Professor Jo Spencer (King’s College London, Department of Immunobiology)

  • Prepared samples for acquisition with Helios mass cytometer (CyTOF), 10X 5’ VDJ with CITE-Seq antibodies, and imaging mass cytometry (Helios).
  • Designed an unsupervised, machine-learning analysis pipeline for analysing tissue-specific B cell subset differences.

Imaging mass cytometry delinates immune cell populations in discoid lupus erythematosus
PI: Dr Thomas J Tull, Professor Jo Spencer (King’s College London, Department of Immunobiology)

  • Prepared skin biopsy slides for acquisition with imaging mass cytometry (Helios) and IHC.
  • Designed an unsupervised, random forest analysis pipeline for analysing and comparing immune cells in DLE and healthy .

2016 – 2021
Cambridge, UK

PhD Dissertation: Lymphocyte subset variability between human lymphoid tissues
Supervised by: Dr. Gavin Pettigrew (University of Cambridge, Department of Surgery), Professor Jo Spencer (King’s College London, Department of Immunobiology)

  • Processed samples from deceased organ donor tissues, prepared samples for acquisition with Helios mass cytometer (CyTOF) and 10X 5’ VDJ with CITE-Seq antibodies, developed a novel biological internal control for normalising batch effects.
  • Designed an unsupervised, machine-learning analysis pipeline for analysing tissue-specific B cell subset differences.
  • Thesis: https://www.repository.cam.ac.uk/handle/1810/324840

2015 – 2016
Vancouver, Canada

Honours BSc Dissertation: Immunological implications of multi-step ALS model
Supervised by: Dr. Neil Cashman (UBC, Centre for Brain Health)

  • Mathematically modeled ALS disease progression by modifying a multi-step disease model, hypothesized a critical step in disease progression variation was sustained immune activation. Validated aspects of the model using in vitro experiments like testing aging effects on microglia activation by misfolded SOD1.

2014 – 2016
Vancouver, Canada

Designing a universal fluorescent reporter of SOD1 protein misfolding
Supervised by: Dr. Neil Cashman (UBC, Centre for Brain Health)

Montreal, Canada

Effects of retinoic acid, iNOS, TNFa on IgA class-switching recombination in vitro
Supervised by: Dr. Jorg Fritz (McGill, Department of Microbiology)

2012- 2014
Vancouver, Canada

Constructed and tested in vitro flagellin-specific chimeric antigen receptors (CARs) for regulatory T cells
Supervised by: Dr. Megan Levings (BC Children’s Hospital Research Institute)

For more details, please refer to the PDF version of my resume above.

Peer-Reviewed Papers

To find me on various literature search engines: PubMed, Google Scholar, ORCID


B cells in human lymphoid structures. Clinical and Experimental Immunology.
Lucia Montorsi, Jacqueline HY Siu, Jo Spencer.
PMID: 36370126. [Full article]

Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and blood. Science Immunology.
Jacqueline HY Siu, Michael J Pitcher, Thomas J Tull, Rebekah L Velounias, William Guesdon, Lucia Montorsi, Krishnaa T Mahbubani, Richard Ellis, Pawan Dhami, Katrina Todd, Ulrich D Kadolsky, Michelle Kleeman, David P D’Cruz, Kourosh Saeb-Parsy, Mats Bemark, Gavin J Pettigrew, Jo Spencer.
PMID: 35302862. [Full article]


Human marginal zone B cell development from early T2 progenitors. Journal of Experimental Medicine.
Thomas J Tull, Michael J Pitcher, William Guesdon, Jacqueline HY Siu, Cristina Lebrero-Fernandez, Yuan Zhao, Nedyalko Petrov, Susanne Heck, Richard Ellis, Pawan Dhami, Ulrich D Kadolsky, Michelle Kleeman, Yogesh Kamra, David J Fear, Susan John, Wayel Jassem, Richard W Groves, Jeremy D Sanderson, Michael G Robson, David P D’Cruz, Mats Bemark, Jo Spencer.
PMID: 33538776. [Full article]


Humoral autoimmunity after solid organ transplantation: germinal ideas may not be natural. Cellular Immunology.
Jacqueline HY Siu, Reza Motallebzadeh, Gavin J Pettigrew.
PMID: 32563029. [Full article]


Human Intestinal Lymphoid Tissue in Time and Space. Mucosal Immunology.
Jo Spencer, Jacqueline HY Siu, Lucia Montorsi.
PMID: 30542110. [Full article]

Prolongation of Allograft Survival by Passenger Donor Regulatory T Cells. American Journal of Transplantation.
Ines G Harper, Olivera Gjorgjimajkoska, Jacqueline HY Siu, Jasvir Parmar, Arend Mulder, Frans HJ Claas, Sarah A Hosgood, Michael L Nicholson, Reza Motallebzadeh, Gavin J Pettigrew.
PMID: 30548563. [Full article]

Reduced CD27 IgD B Cells in Blood and Raised CD27 IgD B Cells in Gut-Associated Lymphoid Tissue in Inflammatory Bowel Disease. Frontiers in Immunology.
Chathyan Pararasa, Na Zhang, Thomas J Tull, Ming HA Chong, Jacqueline HY Siu, William Guesdon, Konstantia Maria Chavele, Jeremy D Sanderson, Louise Langmead, Klaartje Kok, Jo Spencer, Anna Vossenkamper.
PMID: 30891036. [Full article]


Spatiotemporal Segregation of Human Marginal Zone and Memory B Cell Populations in Lymphoid Tissue. Nature Communications.
Yuan Zhao*, Mohamed Uduman*, Jacqueline HY Siu*, Thomas J Tull*, … Steven H Kleinstein, Mats Bemark, Jo Spencer. (* co-primary)
PMID: 30242242. [Full article]

T Cell Allorecognition Pathways in Solid Organ Transplantation. Frontiers in Immunology.
Jacqueline HY Siu, Veena Surendrakumar, James A Richards, Gavin J Pettigrew.
PMID: 30455697. [Full article]

Teaching Experience

2021, 2022

Guest Lecturer. Certificate in Immunology, Institute of Continuing Education, University of Cambridge.
Lectured about transplant immunology, provided additional learning resources, and hosted a “retro journal club” about a milestone transplant rejection paper.

2015 – 2016

Research and teaching assistant. Science One, University of British Columbia.
Designed a new first year biology lab that introduced concepts including DNA extraction, PCR, restriction enzyme digest, DNA gel electrophoresis. Held workshops/weekly office hours and helped when the main instructor was away


The best way to contact me is via email or message me via Twitter.