Jacqueline HY Siu

Jacqueline Siu

About

I am a fourth-year PhD candidate in the Department of Surgery (Transplantation) at the University of Cambridge where I am supervised by Dr. Gavin Pettigrew and Professor Jo Spencer (King’s College London).

My research, funded by a Gates Cambridge scholarship, combines traditional experimental immunology techniques with computational biology to understand tissue-specific differences in lymphocytes. In particular, I have been using single cell datasets (mass cytometry and 10X) to investigate the mucosal immune system in human secondary lymphoid organs during immune homeostasis.

I am also passionate about public engagement, STEM outreach, and mentoring. You can contact me about consulting work and public engagement via email.

Research Projects

2016 – present
Cambridge, UK

PhD Dissertation: Tissue-specific, regional lymphocyte differences in human lymphoid tissues
Supervised by: Dr. Gavin Pettigrew (University of Cambridge, Department of Surgery), Professor Jo Spencer (King’s College London, Department of Immunobiology)

  • Processed samples from deceased organ donor tissues, prepared samples for acquisition with Helios mass cytometer (CyTOF) and 10X 5’ VDJ with CITE-Seq antibodies, developed a novel biological internal control for normalising batch effects.
  • Designed an unsupervised, machine-learning analysis pipeline for analysing tissue-specific B cell subset differences.
  • Co-first author to a Nature Communications paper

2015 – 2016
Vancouver, Canada

Honours BSc Dissertation: Immunological implications of multi-step ALS model
Supervised by: Dr. Neil Cashman (UBC, Centre for Brain Health)

  • Mathematically modeled ALS disease progression by modifying a multi-step disease model, hypothesized a critical step in disease progression variation was sustained immune activation. Validated aspects of the model using in vitro experiments like testing aging effects on microglia activation by misfolded SOD1.

2014 – 2016
Vancouver, Canada

Designing a universal fluorescent reporter of SOD1 protein misfolding
Supervised by: Dr. Neil Cashman (UBC, Centre for Brain Health)

2014
Montreal, Canada

Effects of retinoic acid, iNOS, TNFa on IgA class-switching recombination in vitro
Supervised by: Dr. Jorg Fritz (McGill, Department of Microbiology)

2012- 2014
Vancouver, Canada

Constructed and tested in vitro flagellin-specific chimeric antigen receptors (CARs) for regulatory T cells
Supervised by: Dr. Megan Levings (BC Children’s Hospital Research Institute)

For more details, please refer to the PDF version of my resume above.

Peer-Reviewed Papers

To find me on various literature search engines: PubMed, Google Scholar, ORCID

2018

Spatiotemporal Segregation of Human Marginal Zone and Memory B Cell Populations in Lymphoid Tissue. Nature Communications.
Yuan Zhao*, Mohamed Uduman*, Jacqueline HY Siu*, Thomas J Tull*, … Steven H Kleinstein, Mats Bemark, Jo Spencer. (* co-primary)
PMID: 30242242. [Full article]

T Cell Allorecognition Pathways in Solid Organ Transplantation. Frontiers in Immunology.
Jacqueline HY Siu, Veena Surendrakumar, James A Richards, Gavin J Pettigrew.
PMID: 30455697. [Full article]

2019

Human Intestinal Lymphoid Tissue in Time and Space. Mucosal Immunology.
Jo Spencer, Jacqueline HY Siu, Lucia Montorsi.
PMID: 30542110. [Full article]

Prolongation of Allograft Survival by Passenger Donor Regulatory T Cells. American Journal of Transplantation.
Ines G Harper, Olivera Gjorgjimajkoska, Jacqueline HY Siu, Jasvir Parmar, Arend Mulder, Frans HJ Claas, Sarah A Hosgood, Michael L Nicholson, Reza Motallebzadeh, Gavin J Pettigrew.
PMID: 30548563. [Full article]

Reduced CD27 IgD B Cells in Blood and Raised CD27 IgD B Cells in Gut-Associated Lymphoid Tissue in Inflammatory Bowel Disease. Frontiers in Immunology.
Chathyan Pararasa, Na Zhang, Thomas J Tull, Ming HA Chong, Jacqueline HY Siu, William Guesdon, Konstantia Maria Chavele, Jeremy D Sanderson, Louise Langmead, Klaartje Kok, Jo Spencer, Anna Vossenkamper.
PMID: 30891036. [Full article]

2020

Humoral autoimmunity after solid organ transplantation: germinal ideas may not be natural. Cellular Immunology.
Jacqueline HY Siu, Reza Motallebzadeh, Gavin J Pettigrew.
PMID: 32563029. [Full article]

Contact

The best way to contact me is via email or message me via Twitter.